Academic Radiology
Volume 14, Issue 7 , Pages 859-870 , July 2007

In Vitro Effect of Focused Ultrasound or Thermal Stress on HSP70 Expression and Cell Viability in Three Tumor Cell Lines

  • Walter Hundt, MD

      Affiliations

    • Department of Radiology, Lucas MRS Research Center, Stanford School of Medicine, Stanford, CA
    • Department of Clinical Radiology, University of Munich, LMU Munich, Marchioninistrasse 15, 81377 Munich, Germany
    • Corresponding Author InformationAddress correspondence to: W.H.
  • ,
  • Caitlin E. O’Connell-Rodwell, PhD

      Affiliations

    • Department of Pediatrics, Microbiology & Immunology and Radiology, Stanford School of Medicine, Stanford, CA
  • ,
  • Mark D. Bednarski, MD, PhD

      Affiliations

    • Department of Radiology, Lucas MRS Research Center, Stanford School of Medicine, Stanford, CA
  • ,
  • Silke Steinbach, MD

      Affiliations

    • Department of Otolaryngology Head and Neck Surgery, Technical University of Munich, Germany.
  • ,
  • Samira Guccione, PhD

      Affiliations

    • Department of Radiology, Lucas MRS Research Center, Stanford School of Medicine, Stanford, CA

Received 5 December 2006 ,Accepted 13 April 2007.

References 

  1. Damianou C, Hynynen K. The effect of various physical parameters on the size and shape of necrosed tissue volume during ultrasound surgery. J Acoust Soc Am. 1994;95:1641–1649
  2. McDannold NJ, Jolesz FA, Hynynen KH. Determination of the optimal delay between sonications during focused ultrasound surgery in rabbits by using MR imaging to monitor thermal buildup in vivo. Radiology. 1999;211:419–426
  3. Mitragotri S, Blankschtein D, Langer R. Ultrasound-mediated transdermal protein delivery. Science. 1995;269:850–853
  4. Huber PE, Pfisterer P. In vitro and in vivo transfection of plasmid DNA in the Dunning prostate tumor R3327-AT1 is enhanced by focused ultrasound. Gene Ther. 2000;7:1516–1525
  5. Huber PE, Mann MJ, Melo LG, et al. Focused ultrasound (HIFU) induces localized enhancement of reporter gene expression in rabbit carotid artery. Gene Ther. 2003;10:1600–1607
  6. Lagneaux L, de Meulenaer EC, Delforge A, et al. Ultrasonic low-energy treatment: a novel approach to induce apoptosis in human leukemic cells. Exp Hematol. 2002;30:1293–1301
  7. Honda H, Zhao QL, Kondo T. Effects of dissolved gases and an echo contrast agent on apoptosis induced by ultrasound and its mechanism via the mitochondria caspase pathway. Ultrasound Med Biol. 2002;28:673–682
  8. Vykhodtseva N, McDannold N, Martin H, et al. Apoptosis in ultrasound-produced threshold lesions in the rabbit brain. Ultrasound Med Biol. 2001;27:111–117
  9. Huber PE, Jenne JW, Rastert R, et al. A new noninvasive approach in breast cancer therapy using magnetic resonance imaging-guided focused ultrasound surgery. Cancer Res. 2001;61:8441–8447
  10. McDannold N, Vykhodtseva N, Hynynen K. Targeted disruption of the blood-brain barrier with focused ultrasound: association with cavitation activity. Phys Med Biol. 2006;51:793–807
  11. Dreano M, Brochot J, Myers A, et al. High-level, heat-regulated synthesis of proteins in eukaryotic cells. Gene. 1986;49:1–8
  12. Guilhon E, Voison P, de Zwart JA, et al. Spatial and temporal control of transgene expression in vivo using a heat-sensitive promoter and MRI-guided focused ultrasound. J Gene Med. 2003;5:333–342
  13. Altmeyer A, Maki RG, Feldweg AM, et al. Tumor-specific cell surface expression of the—KDEL containing, endoplasmic reticular heat shock protein gp96. Int J Cancer. 1996;69:340–349
  14. Arispe N, DeMaio A. ATP and ADP modulate a cation channel formed by Hsp70 in acidic phospholipid membranes. J Biol Chem. 2000;275:30839–30843
  15. DeNagel DC, Pierce SK. A case for chaperones in antigen processing. Immunol Today. 1992;3:86–89
  16. Hartl FU. Molecular chaperones in protein folding. Nature. 1996;381:571–580
  17. Hendrick JP, Hartl FU. Molecular chaperone functions of heat-shock proteins. Annu Rev Biochem. 1993;62:349–384
  18. Milarski KL, Morimoto RI. Expression of human HSP70 during the synthetic phase of the cell cycle. Proc Natl Acad Sci U S A. 1986;83:9517–9521
  19. Morimoto RI, Tissieres A, Georgopoulos C. The stress response, function of the proteins, and perspectives. In:  Morimoto RI,  Tissieres A,  Georgopoulos C editor. Stress proteins in biology and medicine. New York, NY: Cold Spring Harbor Laboratory Press; 1990;p. 1–36
  20. Hightower LE. Heat shock, stress proteins, chaperones, and proteotoxicity. Cell. 1991;66:191–197
  21. Contag CH, Bachmann MH. Advances in in vivo bioluminescence imaging of gene expression. Annu Rev Biomed Eng. 2002;4:235–260
  22. Hooper CE, Ansorge RE, Rushbrooke JG. Low-light imaging technology in the life sciences. J Biolumin Chemilumin. 1994;9:113–122
  23. Contag CH, Spilman SD, Contag PR, et al. Visualizing gene expression in living mammals using a bioluminescent reporter. Photochem Photobiol. 1997;66:523–531
  24. Contag PR, Olomu IN, Stevenson DK, et al. Bioluminescent indicators in living mammals. Nat Med. 1998;4:245–247
  25. Guilhon E, Voisin P, de Zwart JA, et al. Spatial and temporal control of transgene expression in vivo using a heat-sensitive promoter and MRI-guided focused ultrasound. J Gene Med. 2003;5:333–342
  26. Rome C, Couillaud F, Moonen CTW. Spatial and temporal control of expression of therapeutic genes using heat shock protein promoters. Methods. 2005;35:188–198
  27. Abdollahi A, Domhan S, Jenne JW, et al. Apoptosis signals in lymphoblasts induced by focused ultrasound. FASEB J. 2004;18:1413–1414
  28. Huber PE, Debus J. Tumor cytotoxicity in vivo and radical formation in vitro depend on the shock wave-induced cavitation dose. Radiat Res. 2001;156:301–309
  29. Debus J, Spoo J, Jenne J, et al. Sonochemically induced radicals generated by pulsed high-energy ultrasound in vitro and in vivo. Ultrasound Med Biol. 1999;25:301–306
  30. Suslick KS, Flint EB. Sonoluminescence from non-aqueous liquids. Nature. 1987;330:553–555
  31. Simko M, Hartwig C, Lantowa M, et al. Hsp70 expression and free radical release after exposure to non-thermal radio-frequency electromagnetic fields and ultrafine particles in human Mono Mac 6 cells. Toxicol Lett. 2006;161:73–82
  32. Barnett SB, ter Haar GR, Ziskin MC, et al. Current status of research on biophysical effects of ultrasound. Ultrasound Med Biol. 1994;20:205–218
  33. Huber PE, Jenne J, Debus J, Wannenmacher MF, Pfisterer P, et al. A comparision of shock wave and sinusoidal-focused ultrasound-induced localized transfection of HeLa cells. Ultrasound Med Biol. 1999;25:1451–1457
  34. Thuroff S, Chaussy C, Vallancien G, et al. High-intensity focused ultrasound and localized prostate cancer: efficacy results from the European multicentric study. J Endourol. 2003;17:673–677
  35. Wu F, Wang ZB, Cao YD, et al. A randomised clinical trial of high-intensity focused ultrasound ablation for the treatment of patients with localised breast cancer. Br J Can. 2003;89:2227–2233
  36. Kennedy JE, Wu F, ter Haar GR, et al. High-intensity focused ultrasound for the treatment of liver tumours. Ultrasonics. 2004;42:931–935
  37. Huber PE, Pfisterer P. In vitro and in vivo transfection of plasmid DNA in the Dunning prostate tumor R3327-AT1 is enhanced by focused ultrasound. Gene Therapy. 2000;7:1516–1525
  38. Huber PE, Jenne J, Debus J, et al. A comparison of shock wave ans sinusoidal-focused ultrasound induced localized transfection of HeLa Cells. Ultrasound Med Biol. 1999;25:1451–1457
  39. Frenkel V, Li KC. Potential role of pulsed-high intensity focused ultrasound in gene therapy. Future Oncol. 2006;2:111–119
  40. Vekris A, Maurange C, Moonen C, et al. Control of transgene expression using local hyperthermia in combination with a heat sensitive promoter. J Gene Med. 2000;2:89–96
  41. Lohr F, Hu K, Huang Q, et al. Enhancement of radiotherapy by hyperthermia-regulated gene therapy. Int J Radiat Oncol Biol Phys. 2000;48:1513–1518
  42. Huang Q, Hu JK, Lohr F, et al. Heat-induced gene expression as a novel targeted cancer gene therapy strategy. Cancer Res. 2000;60:3435–3439
  43. Gething MJ, Sambrook J. Protein folding in the cell. Nature. 1992;355:33–45
  44. Henle KJ, Nagle WA. Inhibition of heat shock protein synthesis and protein glycosylation by stepdown heating. Exp Cell Res. 1991;196:184–191
  45. O’Connell-Rodwell CE, Shriver D, Simanovskii D, et al. A genetic reporter of thermal stress defines physiologic zones over a defined temperature range. FASEB J. 2004;18:264–271

 Supported in part by the Lucas Foundation and the Phil Allen Trust.

PII: S1076-6332(07)00200-0

doi: 10.1016/j.acra.2007.04.008

Academic Radiology
Volume 14, Issue 7 , Pages 859-870 , July 2007