Longitudinal Assessment of Colonic Tumor Fate in Mice by Computed Tomography and Optical Colonoscopy

Durkee, Benjamin Y.; Shinki, Kazuhiko; Newton, Michael A.; Iverson, Caitlin E.; Weichert, Jamey P.; Dove, William F.; Halberg, Richard B.

doi:10.1016/j.acra.2009.07.023

« Previous Next »Academic Radiology
Volume 16, Issue 12 , Pages 1475-1482, December 2009

Longitudinal Assessment of Colonic Tumor Fate in Mice by Computed Tomography and Optical Colonoscopy

Benjamin Y. Durkee, PhD
- Department of Medical Physics, University of Wisconsin – Madison, 1400 University Avenue, Madison, WI 53706
Kazuhiko Shinki, PhD
- Department of Radiology, University of Wisconsin – Madison, 1400 University Avenue, Madison, WI 53706
Michael A. Newton, PhD
- Department of Biostatistics and Medical Informatics and Department of Statistics, University of Wisconsin – Madison, 1400 University Avenue, Madison, WI 53706
Caitlin E. Iverson, BS
- McArdle Laboratory for Cancer Research – Department of Oncology, University of Wisconsin – Madison, 1400 University Avenue, Madison, WI 53706
Jamey P. Weichert, PhD
- Department of Medical Physics, University of Wisconsin – Madison, 1400 University Avenue, Madison, WI 53706
- Department of Radiology, University of Wisconsin – Madison, 1400 University Avenue, Madison, WI 53706
William F. Dove, PhD
- McArdle Laboratory for Cancer Research – Department of Oncology, University of Wisconsin – Madison, 1400 University Avenue, Madison, WI 53706
- Laboratory of Genetics, University of Wisconsin – Madison, 1400 University Avenue, Madison, WI 53706
- Address correspondence to: W.F.D.
Richard B. Halberg, PhD
- McArdle Laboratory for Cancer Research – Department of Oncology, University of Wisconsin – Madison, 1400 University Avenue, Madison, WI 53706
- Address correspondence to: R.B.H.

Received 7 May 2009; accepted 23 July 2009.

Rationale and Objectives

The purpose of this study was to evaluate the relative merits of micro-computed tomograph colonography (mCTC) and optical colonoscopy (OC) for longitudinal studies of colonic tumors in mice.

Materials and Methods

Colonic tumors in mice carrying the Min allele of Apc were followed over several weeks using mCTC and OC. A total of 146 colonic tumors were monitored: 62 in 32 untreated Min mice, 53 in 43 Min mice treated with 5-fluorouracil (5-FU), and 31 in 17 Min mice treated with piroxicam.

Results

Colonic tumors in Min mice had three different spontaneous fates: 29 grew, 24 remained static, and 9 regressed. Treating Min mice with 5-FU increased the percentage of regressing tumors from 15% to 58%. The response was dependent in part on the initial size of the tumor. By contrast, treating Min mice with piroxicam did not alter colonic tumor fate.

Conclusions

mCTC and OC can be used to determine the spontaneous fates of colonic tumors in mice and to document their individual responses to treatment. The ability to follow individually annotated colonic tumors reduces the number of mice needed for testing.

Key Words: Mouse models of human colorectal cancer, microCT colonography, optical colonoscopy, longitudinal studies, tumor fate mapping

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Supported by funds to Jamey Weichert from the University of Wisconsin Comprehensive Cancer Center, grants R37CA63677and U01CA84227 to William F. Dove from the National Cancer Institute, grant R01 CA64364 to Michael A. Newton from the National Cancer Institute, and grant U19CA113297–03 to Ben Shen from the National Cancer Institute NCDDG Program.

PII: S1076-6332(09)00427-9

doi:10.1016/j.acra.2009.07.023

« Previous Next »Academic Radiology
Volume 16, Issue 12 , Pages 1475-1482, December 2009

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