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Series of intravoxel incoherent motion and T2* magnetic resonance imaging mapping in detection of liver perfusion changes and regeneration among partial hepatectomy in Sprague-Dawley rats

      Rationale and objectives

      To evaluate liver perfusion changes and their effect on liver regeneration (LR) after partial hepatectomy (PH) using intravoxel incoherent motion (IVIM) and T2* mapping in a rat model.

      Methods

      One hundred and two rats underwent 30%, 50%, or 70% PH. Within each group (n = 34), rats in MR imaging subgroup (n = 10) underwent liver IVIM and T2* mapping before and within 2 h, 1, 2, 3, 5, 7, 14, and 21 days post-PH to measure D*, perfusion fraction (PF), and T2* values. Three rats from histologic subgroup (n = 24) sacrificed at each time point for hepatocyte Ki-67 indices and diameters measurement.

      Results

      Liver D* and PF values decreased immediately post-PH, then returned to original level as LR progressed in all groups. PF values in 70% PH group were significantly lower than in the other two groups (p < .05). D* and PF values correlated significantly with hepatocyte Ki-67 indices (r =  –0.588 to –0.915; p < .05) and hepatocyte diameter (r  = –0.555 to –0.792; p < .05). Liver T2* values decreased immediately within 2 h post-PH, then increased to a high level and followed with returning to original level gradually. The duration of the high T2* levels was consistent with Ki-67 indices.

      Conclusions

      Liver perfusion decreased immediately followed with increasing gradually after PH. IVIM and T2* mapping are promising methods for monitoring changes of liver perfusion. IVIM-derived D* value is the best indicator in reflecting the process of LR noninvasively.

      Keywords

      Abbreviations:

      FOV (field of view), ICC (intraclass correlation coefficient), IVIM (intravoxel incoherent motion), LR (liver regeneration), MRI (magnetic resonance imaging), PF (perfusion fraction), PH (partial hepatectomy), ROI (region of interest), SD (standard deviation), TE (echo time), TR (repetition time), WI (weighted imaging)
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