Rationale and Objectives
The purpose of this study was to explore the feasibility of magnetic resonance imaging-guided
focused ultrasound surgery (MRgFUS) for the treatment of an adenomyosis model of Bama
pigs and the changes in the level of oxytocin receptor (OTR), vascular endothelial
growth factor (VEGF), and cyclooxygenase-2 (COX-2) in the myometrium tissues of Bama
pigs after MRgFUS.
Materials and Methods
Three Bama pig models of adenomyosis were established by autologous endometrial implantation
and evaluated by magnetic resonance imaging, computed tomography, and hematoxylin-eosin
(H&E) staining. After the successful construction of the model, the pigs underwent
MRgFUS. Before the modeling surgery, three months after the modeling, and two months
after ablation, the myometrium tissues were clipped, then embedded and H&E stained
for immunohistochemical examination. The average optical density of OTR, VEGF, and
COX-2 were semi-quantitatively analyzed.
Results
The adenomyosis models were established in all Bama pigs and confirmed by magnetic
resonance imaging, computed tomography and H&E staining. Magnetic resonance imaging
and computed tomography examination showed that the uterine wall at the modeling site
was significantly thickened with uneven enhancement after contrast injection. All
Bama pigs with adenomyosis lesions underwent MRgFUS without complications. The expression
level of OTR and COX-2 in the myometrium increased three months after modeling surgery
and decreased two months after MRgFUS. The expression level of VEGF decreased two
months after MRgFUS.
Conclusion
Autologous endometrial implantation is effective in establishing the adenomyosis model
of Bama pigs. It is feasible to treat adenomyosis in the Bama pig model with MRgFUS.
The levels of OTR, COX-2 and VEGF in the local myometrium decreased after MRgFUS,
which may be associated with symptom relief after treatment.
Key Words
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Article info
Publication history
Published online: January 07, 2023
Accepted:
November 30,
2022
Received in revised form:
November 15,
2022
Received:
July 19,
2022
Publication stage
In Press Corrected ProofIdentification
Copyright
© 2022 The Association of University Radiologists. Published by Elsevier Inc. All rights reserved.
